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目的:检测孕中期双胎妊娠孕妇血清甲胎蛋白(AFP)、人绒毛膜促性腺激素β亚基(β-hCG)和游离雌三醇(uE3)的浓度,通过文献上的校正方法得到校正后MoM值[1],计算唐氏征和神经管缺陷风险,通过与单胎标本的比较,评判该方法是否适合本实验室。方法:收集双胎妊娠109例,孕中期(14周～21周)采集孕妇静脉血,用化学发光免疫分析技术测定血清AFP、hCG和uE3的浓度,结合孕妇信息计算风险,并于分娩后随访,确定胎儿妊娠结局。以同期正常单胎妊娠的10829例作对照组。结果:校正后109例双胎妊娠孕妇血清的MoM中值是AFP 0.92,hCG 1.01,uE30.98,AFP的校正结果与文献上略有差异,hCG和uE3基本吻合。结论:鉴于双胎妊娠的发生概率较小,可以采用文献上校正参数,但当累积的样本足够多时,可以适当调整该参数。
OBJECTIVE: To detect the concentrations of serum alpha-fetoprotein (AFP), human chorionic gonadotropin beta subunit (beta-hCG) and free oestriol (uE3) in the second trimester of pregnancy, and to correct them by the corrective methods in the literature After MoM value [1], Down’s syndrome and neural tube defects were calculated and compared with single-fetus specimens to judge whether the method is suitable for our laboratory. Methods: 109 pregnant women with twin pregnancy were collected and the pregnant women were collected during the second trimester of pregnancy (14 weeks to 21 weeks). The levels of serum AFP, hCG and uE3 were measured by chemiluminescence immunoassay. The risk was calculated according to the information of pregnant women. , To determine the fetal pregnancy outcome. In the same period normal singleton pregnancy 10829 as a control group. Results: The corrected MoM values of 109 pregnant women with twin pregnancies were AFP 0.92, hCG 1.01 and uE30.98, and the corrected results of AFP were slightly different from those in the literature. The correlation between hCG and uE3 was basically consistent. CONCLUSIONS: Given the small probability of twin pregnancies, literature-based calibration parameters can be used, but this parameter can be adjusted appropriately when the cumulative sample is sufficient.