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目的：探讨农药丙溴磷对正常人红细胞的毒性效应。方法：采用Ｂｉａｌｓｃｈｅ试剂法检测红细胞膜唾液酸（ＲＢＣｍ－ＳＡ），化学比色法测定红细胞膜Ｃａ２＋－ＡＴＰ酶和全血中谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）。结果：丙溴磷浓度在２×１０－４ｍｏｌ／Ｌ时，可明显降低ＲＢＣｍ－ＳＡ含量，并呈剂量－反应关系。丙溴磷浓度在２×１０－３ｍｏｌ／Ｌ时，红细胞膜Ｃａ２＋－ＡＴＰ酶和全血ＧＳＨ－Ｐｘ明显受抑制，其受抑制程度基本同ＲＢＣｍ－ＳＡ降低呈平行关系。结论：丙溴磷对红细胞的毒性效应与ＲＢＣｍ－ＳＡ的代谢障碍有关。
Objective: To investigate the toxicity of pesticide profenofos in normal human erythrocytes. Methods: RBCm-SA was detected by Bialsche’s reagent method. GSH-Px in whole blood and Ca2 + -ATPase in erythrocyte membrane were determined by chemical colorimetry. Results: Bromosulfate concentration of 2 × 10-4mol / L, can significantly reduce the RBCm-SA content, and the dose-response relationship. Bromosulfate concentration of 2 × 10-3mol / L, the erythrocyte membrane Ca2 + -ATPase and whole blood GSH-Px significantly inhibited the degree of inhibition was basically parallel with RBCm-SA decreased. Conclusion: The toxicity of profenofos to erythrocytes is related to the metabolic disorder of RBCm-SA.