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A sensitive HPLC method for the detection of amino acids with pre-columnfluorescence derivatization with carbazole-9-yl-acetyl chloride has been developed. This method,in conjunction with a multi-gradient program, offers baseline resolution of the common CRAC-amino acids from a linear acetonitrile gradient. Separation of amino acid derivatives was obtainedon a reversed-phase C18 column. Derivatization and chromatographic conditions were optimized.Amino acid derivatives were eluted in 40 min with good reproducibility. The relative standarddeviations (n=6) at an analytical concentration of 100 pmol are < 4%. The methods described arealso suitable for the analysis of amino acids in different biological samples.
A sensitive HPLC method for the detection of amino acids with pre-column fluorescence derivatization with carbazole-9-yl-acetyl chloride has been developed. This method, in conjunction with a multi-gradient program, offers baseline resolution of the common CRAC-amino acids from a linear acetonitrile gradient. Separation of amino acid derivatives was obtained from a reversed phase C18 column. Derivatization and chromatographic conditions were optimized. Amino acid derivatives were eluted in 40 min with good reproducibility. The relative standarddeviations (n = 6) at an analytical concentration of 100 pmol are <4%. The methods described arealso suitable for the analysis of amino acids in different biological samples.