This work aimed for pyrite separation from Abu Tartur black shale as a source of sulfur to be an added economic value of Abu Tartur area. The considered samples in the present work were collected from a core drilled in Abu Tartur plateau representing the pyrite-rich black shale of the U. Cretaceous age.Sample characterization was carried out using petrographic microscope, XRD, DTA/DTG, C/S and XRF techniques. Clay minerals, silt-sized quartz, calcite, and hematite were the main minerals associating pyrite(5.34%). Liberation behavior of the sample was about 80% below 5 lm.Sample processing was achieved through one-day soaking followed by classification using 1 inch Mozley hydro-cyclone where about 35.5% by weight went to underflow and 64.5% went to overflow.The underflow product was subjected to an advanced gravity separation process using SB-40 Falcon Concentrator through a CCD statistical design prepared by Design-Expert 6.0 software proposed to optimize the separation process through a study for the effects of frequency(Hz) and water pressure(Psi) on both assay and recovery of the sulfur-rich heavy fraction.A heavy concentrate weighed 10.90% with inorganic sulfur content reached 11.37%(21.24% pyrite) with overall recovery(50.01%) was obtained after two cleaning at the optimum conditions. This work aimed for pyrite separation from Abu Tartur black shale as a source of sulfur to be an added economic value of Abu Tartur area. The considered samples were the work done collected from a core drilled in Abu Tartur plateau representing the pyrite-rich black shale of the U. Cretaceous age.Sample characterization was carried out using petrographic microscope, XRD, DTA / DTG, C / S and XRF techniques. Clay minerals, silt-sized quartz, calcite, and hematite were the main minerals associating pyrite (5.34 %). Liberation behavior of the sample was about 80% below 5 lm. Example was was through one-day soaking followed by classification using 1 inch Mozley hydro-cyclone where about 35.5% by weight went to underflow and 64.5% went to overflow The underflow product was subjected to an advanced gravity separation process using SB-40 Falcon Concentrator through a CCD statistical design prepared by Design-Expert 6.0 software proposed to optimize the separation process through a study for the effects of frequency (Hz) and water pressure (Psi) on both assay and recovery of the sulfur-rich heavy fraction. A heavy concentrate weighed 10.90% with inorganic sulfur content reached 11.37% (21.24% pyrite) with overall recovery (50.01%) was obtained after two cleaning at the optimum conditions.