HLA-DR分型以及某些临床免疫学检查需要获得B淋巴细胞。使用尼龙棉柱分离是一个简便的方法。过去一般使用50～100mg左右的尼龙棉制成6cm高的尼龙棉柱。但我们两年的经验表明该方法需要血液量较多,B细胞纯度欠高。本文对此方法改进,取得满意结果。 材料和方法 一、磷酸缓冲液(PBS):(1)贮存液:NaCl 216g,KCl 7g,KH_2PO_4 6g,Na_2HPO_4 HLA-DR typing and some clinical immunological tests require B lymphocytes. The use of nylon cotton column separation is a simple way. In the past generally use about 50 ~ 100mg made of nylon cotton 6cm high nylon cotton column. However, our two years of experience have shown that this method requires more blood and less pure B cells. In this paper, this method is improved, and satisfactory results are obtained. Materials and methods First, phosphate buffer solution (PBS): (1) stock solution: NaCl 216g, KCl 7g, KH_2PO_4 6g, Na_2HPO_4