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本文采用聚合酶链式反应（PCR），结合银染单链构型多态分析（SSCP）技术，取代同位素掺入放射自显影方法，检测了16例胰腺癌P53基因外显子（exon．E）4－8的改变。材料和方法一、癌组织采集和DNA样品的制备：（1）胰腺癌标本取自手术切除或取做病理检查的癌组织约1克。术中证
In this paper, 16 cases of pancreatic cancer P53 gene exons (exon.E) were detected by polymerase chain reaction (PCR) combined with silver staining single-strand conformation polymorphism analysis (SSCP) technique instead of isotope-incorporated autoradiography. ) Changes of 4-8. Materials and Methods 1. Collection of cancerous tissues and preparation of DNA samples: (1) Pancreatic cancer specimens were taken from 1 g of cancerous tissue removed surgically or taken for pathological examination. Intraoperative