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金色叙利亚仓鼠侧腹器官模型已被认为是检测抗雄激素的理想手段,因其毛囊皮脂腺的解剖学特征以及对激素刺激的反应与人相似.作者设计了特异定量测定靶组织反应的方法.雌仓鼠,12周龄,每天光照12小时,以戊巴比妥(80～140mg i.p.)麻醉,将1cm长的含睾酮(T)的硅化胶囊植入腹侧颈部皱折皮下,测量色素斑的最大纵、横直径,以两者乘积为指标;毛细管穿刺内眶静脉丛取血,放射免疫法测定血清中T含量.以上检测从实验开始作记录,每周一次,持续6周.用相同条件的雄鼠作对照.在0,3,6周处死雌鼠,取侧腹器官及周围数毫米的皮肤作常规切片,测量皮脂数量、面积及毛囊最小直径.用药2周后色素斑明显增大,4周达到高峰,至6周下降26.7%,血清T浓度也在4周达到高峰,但两者无相关性(P>0.05).雄鼠两指标的最大值均在第1周,以后逐步下降37.4%,两者具有相关
The golden Syrian hamster flank organ model has been considered as an ideal means of detecting anti-androgens because of the anatomical features of the hair follicle sebaceous glands and the response to hormone stimulation similar to humans. The authors designed a method for specific quantitative determination of target tissue responses. Hamsters, 12 weeks old, were exposed to light for 12 hours daily and anesthetized with pentobarbital (80-140 mg ip). Silicified capsules containing testosterone (T) 1 cm long were subcutaneously implanted into the ventral neck folds and pigmented plaques were measured The maximum vertical and horizontal diameter, the product of the two as an indicator; capillary perforation within the orbital venous plexus blood, radioimmunoassay T level in serum.This test was recorded from the beginning of the experiment, once a week for 6 weeks.Using the same conditions Males were sacrificed at weeks 0, 3, and 6, and the flaps of the flank organs and surrounding several millimeters of skin were cut into sections to measure the number and area of sebum and the minimum diameter of hair follicles.After 2 weeks of treatment, pigmentation was significantly increased , Peaked at 4 weeks, decreased 26.7% at 6 weeks and peaked at 4 weeks, but no correlation was found between the two groups (P> 0.05). The maximum values of both indexes in male rats were in the first week and then gradually Down 37.4%, the two are related