论文部分内容阅读
目的:观察肾复康Ⅱ号胶囊对大鼠肾间质纤维化及转化生长因子-β1的表达情况的影响。方法:采用单侧输尿管结扎的方法制造大鼠肾间质纤维化模型。90只SD大鼠随机分为假手术组(对照组)、模型组、肾复康Ⅱ号2.25g/kg组、4.5g/kg组、9g/kg组,肾复康Ⅱ号胶囊各组分别以以上剂量水溶剂灌胃,假手术组及模型组每天同时间以等体积的蒸馏水灌胃。给药7、14、21天后分别将造模大鼠的肾脏进行HE、Masson染色,观察肾间质纤维化的面积的变化;通过免疫组化的方法检测肾间质中转化生长因子-β1的表达情况。结果:模型组和肾复康Ⅱ号胶囊各组均出现肾间质纤维化病理改变,各剂量组(有效剂量2.25g/kg～9g/kg)病变程度与模型组相比较轻,具有显著性差异。肾复康Ⅱ号胶囊各组之间无显著性差异。TGF-β1表达在模型组和肾复康Ⅱ号胶囊组明显强于假手术组,肾复康Ⅱ号胶囊各组(有效剂量2.25g/kg～9g/kg)TGF-β1表达较模型组明显减少。结论:肾复康Ⅱ号胶囊可以有效抑制肾间质纤维化及转化生长因子-β1的表达,这可能是益肾散结法抗肾间质纤维化的部分途径。
Objective: To observe the effects of Shenfukang Ⅱ capsules on renal interstitial fibrosis and the expression of transforming growth factor-β1 in rats. Methods: Rat model of renal interstitial fibrosis was made by unilateral ureteral ligation. 90 SD rats were randomly divided into sham operation group (control group), model group, Shenfukang Ⅱ 2.25g / kg group, 4.5g / kg group, 9g / kg group and Shenfukang Ⅱ capsule respectively The above dose of water solvent gavage, sham operation group and model group at the same time every day with equal volume of distilled water gavage. The kidneys of the model rats were stained with HE and Masson respectively at 7, 14 and 21 days after administration, and the area of renal interstitial fibrosis was observed. The expression of transforming growth factor-β1 in renal interstitium was detected by immunohistochemistry Express the situation. Results: The pathological changes of renal interstitial fibrosis occurred in model group and Shenfukang Ⅱ capsules. The lesion degree of each dose group (effective dose 2.25g / kg ~ 9g / kg) was lighter than that of model group, with significant difference difference. Shenfukang Ⅱ capsules no significant difference between groups. The expression of TGF-β1 in model group and ShenfukangⅡ capsule group was significantly higher than that in sham-operated group. The expression of TGF-β1 in each group (effective dose 2.25g / kg ~ 9g / kg) cut back. Conclusion: Shenfukang Ⅱ Capsule can effectively inhibit the expression of renal interstitial fibrosis and transforming growth factor-β1, which may be part of the renal interstitial fibrosis Yishen Sanjie method.