论文部分内容阅读
自制牛耳蜗抗原（ＣＣＡｇ）及牛蜗神经抗原（ＣＣＮＡｇ）采用酶联免疫吸附试验（ＥＬＩＳＡ）对５２例（８９侧）不明原因感音神经性聋患者及对照组２０例无耳疾和免疫性疾病的患者进行了检测，结果抗ＣＣＡｇ抗体水平对照组为０．３８１１±０．０７４３，（吸光度Ａ值，ｘ±ｓ，下同），实验组为０．９４２１±０．２４０８，两组差异有高度显著性（Ｐ＜０．０１），以对照组抗ＣＣＡｇ抗体Ａ值的ｘ＋２ｓ判断，实验组２４例抗ＣＣＡＧ抗体阳性，阳性率为４６％。抗ＣＣＮＡｇ抗体水平对照组为０．２２５４±０．０３８２，实验组为０．２３３１±０．０６５６，两组差异无显著性，以ｘ＋２ｓ判断，实验组６例抗ＣＣＮＡｇ抗体阳性，阳性率为１１．５％。认为ＥＬＩＳＡ检测法程序简便，可动态观察患者抗体水平，并能定量分析，配合免疫组织化学方法或免疫转印法可用于内耳自身免疫病的诊断。用ＥＬＩＳＡ法检测到抗ＣＣＮＡｇ抗体，证实了国内外学者的推断，即内耳自身免疫病存在蜗后起源的可能性。
Self-made cochlear antigen (CCAg) and cochlear nerve antigen (CCNAg) in 52 cases (89 sides) of patients with unexplained sensorineural hearing loss and control group, 20 cases without ear disease and immunity Disease patients were tested, the results of anti-CCAg antibody levels in the control group was 0.3811 ± 0.0743 (absorbance A value, x ± s, the same below), the experimental group was 0.9421 ± 0.2408, the difference between the two groups (P <0.01). According to the value of x + 2s of anti-CCAg antibody A in control group, the positive rate of anti-CCAG antibody in experimental group was 24%. The level of anti-CCNAg antibody in the control group was 0.2254 ± 0.0382, and the experimental group was 0.2331 ± 0.0656. There was no significant difference between the two groups. According to x + 2s, the positive rate of anti-CCNAg antibody in the experimental group was 6 .5%. It is considered that the ELISA method has the advantages of simple procedure, dynamic observation of patient antibody level, quantitative analysis, and immunohistochemistry or immunotransfer method for the diagnosis of autoimmune diseases in the inner ear. The anti-CCNAg antibody was detected by ELISA, which confirmed the domestic and foreign scholars’ inference that the origin of cochlea in the inner ear autoimmune disease exists.