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本研究已将从全国各地征集的50份水稻优良种质资源(2n=2x=24)诱变为同源四倍体水稻原种。本文讨论不同处理日期、不同秋水仙碱浓度和处理时间、以及不同品种的抗药性等因素对加倍成功率的影响,结果表明:①不同日期处理水稻幼芽,其加倍成功率不同,4月6日～15日加倍效果最好,加倍成功率达19.4%。②不同浓度秋水仙碱和处理时间其加倍成功率存在一定的差别。0.1%秋水仙碱处理48h 和0.2%处理24h 为佳,其芽恢复率超过58%,加倍成功率超过16%。③不同水稻品种抗药性不同。在所加倍的50个水稻品种中,中作9025抗药性最强,芽恢复率达92.4%,加倍成功率38.7%。盐州14抗药性最差,芽恢复率仅为10.6%。
In this study, 50 rice elite germplasm resources (2n = 2x = 24) collected from all over China were mutated to autotetraploid rice cultivars. The effects of different treatment dates, different colchicine concentration and treatment time, and different varieties of drug resistance on the doubling success rate were discussed in this paper. The results showed that: ① The success rates of different doubling dates were different, Day ~ 15 to double the best, doubling the success rate of 19.4%. ② different concentrations of colchicine and treatment time doubling the success rate there is a certain difference. 0.1% colchicine treatment for 48h and 0.2% is better, the bud recovery rate of more than 58%, doubling the success rate of more than 16%. ③ different varieties of rice resistance. Among the 50 rice varieties doubled, Zhongzuo 9025 had the strongest resistance, the bud recovery rate reached 92.4% and the doubling success rate was 38.7%. Yanzhou 14 worst resistance, bud recovery rate of only 10.6%.