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目的探讨糖皮质激素的应用在大鼠肝移植模型建立中的意义。方法将200只SD大鼠采用简单随机化法随机分为实验组和对照组两组,实验组术前30 min肌肉注射硫酸阿托品溶液0.1 mg/kg、头孢唑啉钠0.3 g/kg及氢化可的松5 mg/kg,对照组术前30 min肌肉注射硫酸阿托品溶液0.1 mg/kg、头孢唑啉钠0.3 g/kg及与激素等量的生理盐水,之后操作步骤2组相同,比较2组间供体手术时间、修肝时间、受体手术时间、无肝期以及7 d内的大鼠存活率。结果实验组和对照组间的供体手术时间、修肝时间、受体手术时间及无肝期比较差异均无统计学意义(P>0.05),而实验组术后第1 d、第3 d、第7 d的成活率均分别明显高于对照组〔96%(48/50)比80%(40/50),P<0.05;92%(46/50)比72%(36/50),P<0.05;90%(45/50)比54%(27/50),P<0.05〕。结论糖皮质激素的使用可能有助于提高肝移植模型大鼠的成活率。
Objective To investigate the significance of glucocorticoid application in the establishment of rat liver transplantation model. Methods 200 SD rats were randomly divided into two groups: experimental group and control group by intramuscular injection of atropine sulfate solution 0.1 mg / kg, cefazolin sodium 0.3 g / kg and hydrogenation Pineal 5 mg / kg, control group 30 minutes before operation intramuscular injection of atropine sulfate solution 0.1 mg / kg, cefazolin sodium 0.3 g / kg and the same amount of saline as the hormone, after the operation of the same two groups, compared with two groups Donor donor surgery time, liver repair time, recipient operation time, anhepatic phase and survival rate of rats within 7 days. Results There were no significant differences in donor operation time, liver repair time, operation time of the recipient and anhepatic phase between the experimental group and the control group (P> 0.05), while the experimental group on the 1st, 3rd , And the survival rates on the 7th day were significantly higher than those in the control group (96% (48/50) vs 80% (40/50), P <0.05; 92% (46/50) vs. 72% , P <0.05; 90% (45/50) vs. 54% (27/50), P <0.05). Conclusion The use of glucocorticoids may help to improve the survival rate of liver transplantation model rats.