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目的探讨血浆对Cyanea capillata水母触手提取物(TOE)溶血活性的影响和孔道形成在其溶血机制中的作用。方法在具有相同红细胞浓度的1%稀释的全血和0.5%红细胞悬液中,检测不同浓度(100、200、400μg/ml)的TOE产生的溶血效应,并在两套体系中分别加入20、50或100 mmol/L的K+、Ca2+、Mg2+、Mn2+、Zn2+、Cu2+、Fe2+、La3+、NH4+等阳离子和离子螯合剂EDTA,观察其对TOE溶血活性的影响。结果在两套溶血检测体系中,TOE均呈现出剂量依赖性的溶血效应,TOE为200μg/ml时在红细胞悬液产生的溶血强度高于稀释的全血;Mn2+、Zn2+、La3+、Cu2+、Fe2+和EDTA均明显降低TOE溶血活性(P<0.05),其中以Zn2+的抑制作用最强,而K+、Ca2+、Mg2+、NH4+可不同程度地增强其溶血活性(P<0.05)。结论血浆对TOE的溶血作用有一定的保护作用,而孔道形成可能在TOE的溶血机制中具有重要作用。
Objective To investigate the effect of plasma on hemolytic activity of Cyanea capillata jellyfish tentacle extract (TOE) and the role of pore formation in its hemolysis mechanism. Methods The hemolytic effects of TOE at different concentrations (100, 200, 400 μg / ml) were detected in 1% diluted whole blood and 0.5% erythrocyte suspensions with the same erythrocyte concentration. Two sets of systems were added with 20, 50 or 100 mmol / L of cation such as K +, Ca2 +, Mg2 +, Mn2 +, Zn2 +, Cu2 +, Fe2 +, La3 +, NH4 + and ion chelator EDTA to observe the effect on TOE hemolytic activity. Results The TOE showed a dose-dependent hemolysis effect in two sets of hemolysis detection systems. When the TOE was 200μg / ml, hemolysis intensity in erythrocyte suspensions was higher than that in diluted whole blood; Mn2 +, Zn2 +, La3 +, Cu2 +, Fe2 + (P <0.05). The inhibitory effect of Zn2 + was the strongest, while K +, Ca2 +, Mg2 + and NH4 + enhanced the hemolytic activity to some extent (P <0.05). Conclusions Plasma has some protective effect on the hemolysis of TOE, and the formation of pore may play an important role in the hemolysis mechanism of TOE.