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建立流式荧光法定量检测神经元特异性烯醇化酶(NSE)的正常参考值,评估其在肺癌诊断中的应用价值。采用流式荧光法检测176例临床血清样本的NSE水平,并与罗氏Elecsys电化学发光法检测结果进行比较,确定两种方法之间的可比性。进一步采用流式荧光法检测668例健康者血清NSE水平,建立健康人群的NSE正常参考值。最后,以流式荧光法检测242例肺癌患者、108例肺部良性疾病者和147例健康者血清NSE水平,评价流式荧光法检测NSE的应用。结果显示,176例临床样本的检测结果表明流式荧光法和电化学发光法相关性良好(Y=0.9746X-0.2075,R2=0.9653),二者具有可比性。根据668例健康者检测结果的第95%百分位数(17.07ng/ml)确定流式荧光法的NSE正常参考值,与罗氏Elec-sys 2010电化学发光法的相似。以NSE>17.07ng/ml判断为阳性,NSE在小细胞肺癌患者中的阳性率显著高于肺鳞癌、肺腺癌患者(P<0.01)。建立了流式荧光法检测NSE的正常参考值,并初步评估了流式荧光法检测NSE在肺癌诊断中的应用,为流式荧光法检测NSE的合理应用和广泛推广奠定了基础。
The normal reference value of neuron-specific enolase (NSE) was quantitatively determined by flow cytometry to evaluate its value in the diagnosis of lung cancer. Flow cytometry was used to detect NSE levels in 176 serum samples and compared with the results of Roche Elecsys electrochemiluminescence assay to determine the comparability between the two methods. Flow cytometric analysis was used to detect NSE levels in 668 healthy volunteers and normal NSE values were established in healthy subjects. Finally, the serum levels of NSE in 242 patients with lung cancer, 108 patients with benign lung diseases and 147 healthy controls were detected by flow cytometry. The application of flow cytometry to detect NSE was evaluated. The results showed that the results of 176 clinical samples showed that there was a good correlation between flow-cytometry and electrochemiluminescence (Y = 0.9746X-0.2075, R2 = 0.9653). The NSE normal reference value for flow cytometry was determined on the 95th percentile (17.07 ng / ml) of 668 healthy controls, similar to the Roche Elec-sys 2010 electrochemiluminescence assay. The positive rate of NSE in patients with small cell lung cancer was significantly higher than that in patients with lung squamous cell carcinoma and lung adenocarcinoma (P <0.01). The normal reference value of flow cytometry (NSF) was established by flow cytometry. The application of flow cytometry to detect NSE in lung cancer was preliminarily evaluated, which laid the foundation for the rational application and widespread promotion of NSE by flow cytometry.