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目的:建立鬼针草茎、叶经微生物发酵前后总黄酮中芦丁、海生菊苷、槲皮素-7-o-β-D-葡萄糖苷和异槲皮苷的HPLC含量测定方法。方法:采用Welch materials-C18柱(4.6 mm×250 mm,5μm),以乙腈(A)-0.2%甲酸水溶液(B)为流动相,A:B=20:80,流速1 m L·min~(-1),检测波长380 nm,柱温25℃。结果:芦丁、海生菊苷、槲皮素-7-o-β-D-葡萄糖苷、异槲皮苷质量浓度分别在1.7~17、1.8~18、1.6~16、2.1~21μg·m L~(-1)内与峰面积呈良好线性关系(r≥0.9994,n=6),方法平均回收率分别为99.63%、99.27%、99.01%、99.22%(RSD≤1.18,n=6)。结论:本方法准确,重复性好,可用于同时测定鬼针草中芦丁、海生菊苷、槲皮素-7-o-β-D-葡萄糖苷和异槲皮苷的含量。
Objective: To establish a HPLC method for the determination of rutin, chrysin, quercetin -7-o-β-D-glucoside and isoquercitrin in the total flavonoids of the stem and leaves of Bidenscharifica before and after microbial fermentation. Methods: The mobile phase consisted of Achorage-C18 column (4.6 mm × 250 mm, 5 μm), acetonitrile-A 0.2% formic acid solution (B) (-1), detection wavelength 380 nm, column temperature 25 ℃. Results: The concentrations of rutin, chrysin, quercetin -7-o-β-D-glucoside and isoquercitrin were 1.7-17, 1.8-18, 1.6-16, 2.1-21μg · m The average recoveries were 99.63%, 99.27%, 99.01%, 99.22% (RSD≤1.18, n = 6) in the range of L ~ (-1) . Conclusion: The method is accurate and reproducible. It can be used for the simultaneous determination of rutin, stevioside, quercetin -7-o-β-D-glucoside and isoquercetin in Bidens.