白喉杆菌毒力的测定,在临床检验的确诊和流行病学带菌者的检查,都属重要。过去,白喉毒力试验均用动物进行,自Elek氏创用“平板法”后,在实验室开辟了一个体外测定白喉毒力的新途径,其优点为方法简单、不需动物,且多认为与动物试验结果相一致。目前已采用此法代替动物,只在平板毒力测定有可疑菌株时,才改用动物法再试一次。关于Elek氏“平板法”所用的培养基及其试验方法,近年来已有若干的研究改进,现综述于下: Determination of diphtheria bacillus virulence in the clinical diagnosis and epidemiological examination of carriers are important. In the past, diphtheria virulence tests were performed on animals. Since the creation of the “plate method” by Elek’s, it has opened up a new way for laboratories to determine the virulence of diphtheria in vitro. The advantages of this method are simple methods, no animals, Consistent with animal test results. This method has been used instead of animals, only in the determination of virulent strains of flat strains, only to use animal law to try again. There have been several research improvements on the medium used for Elek’s “plate method” and its test methods and are summarized below: