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目的 研究联合应用多个DNA位点在尸源鉴定方面的应用价值。方法 应用聚合酶链反应(PCR)、聚丙烯酰胺凝胶电泳分离及银染显带的方法通过对无名尸体的有关检材与可疑双亲或子女进行亲权鉴定。结果 在 80例刑事案件尸源鉴定中 ,38例无名尸体采用较新鲜肌肉 ,通过扩增VNTR、STR多个位点得以判明尸源 ;2 9例采用腐败肌肉、 6例采用骨骼和 2例采用牙齿 ,通过扩增多个STR位点判明了尸源。仅有 1例采用腐败肌肉、 2例采用骨骼未能确定尸源。结论 运用多个位点进行亲权方法可以准确地判明尸源鉴定 ,特别对高度腐败尸体、尸块不全等情况下的尸源鉴定具有很高的实用价值
Objective To study the value of combining multiple DNA sites in the identification of dead bodies. Methods The methods of polymerase chain reaction (PCR), polyacrylamide gel electrophoresis separation and silver staining with banding were used to carry out the paternity test on suspect parents and their relatives. Results In the identification of dead bodies in 80 criminal cases, 38 cases of unnamed corpses were found to be fresher than that of fresh muscle. The corpse was identified by amplifying multiple sites of VNTR and STR, 29 cases of corpulent muscle, 6 cases of bone and 2 cases of Teeth, identified as a corpse source by amplifying multiple STR loci. Only 1 case used corrupt muscle and 2 cases failed to confirm the source of corpse. Conclusion The use of multiple sites for paternity testing can accurately identify the source identification, especially for the corpse of highly corrupt bodies, incomplete body mass identification has a high practical value