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采用PCR法,从水仙(Narcissus tazetta var.chinensis)鳞茎主芽中分离克隆到1个温度诱导脂质运载蛋白基因NtTIL。该基因含有567 bp开放阅读框,编码188个氨基酸。该基因属于lipocalin-2superfamily基因家族;推导的氨基酸序列含有SCR1、SCR2和SCR3 3个植物lipocalin的典型结构域,与小麦、拟南芥等植物的同源性大于75%;编码的蛋白为稳定酸性亲水蛋白,不含有信号肽,进行N末端朝外由外到内的跨膜运动。qRT-PCR结果分析表明,水仙生长发育过程中鳞茎膨大期NtTIL表达量较低;休眠期表达量先升高后降低。推测NtTIL对水仙鳞茎主芽休眠具有一定调控作用。
Using PCR method, a temperature-induced lipocalin gene NtTIL was isolated and isolated from the main bulb of Narcissus tazetta var. Chinensis. The gene contains a 567 bp open reading frame encoding 188 amino acids. The deduced amino acid sequence contains typical domains of lipocalin of SCR1, SCR2 and SCR3. The deduced amino acid sequence is more than 75% homologous to plants such as wheat and Arabidopsis thaliana. The encoded protein is stable acid Hydrophilic protein, does not contain signal peptide, N-terminal outward-to-outward transmembrane movement. The analysis of qRT-PCR results showed that the expression of NtTIL in bulblet stage was lower during the growth and development of daffodils, and the expression level in dormant stage increased first and then decreased. Speculated that NtTIL on daffodil bulb bud dormancy has a certain regulatory role.