大量的丝虫幼虫是进行人体丝虫病免疫学研究的必备条件,遗憾的是罗阿和常现丝虫因未建立适宜的实验室内生活史,极大地妨碍了这两种丝虫病免疫学研究的进展。作者采用简便的不连续流动式离心法,从高度感染病人的周围血中收集微丝蚴,标本凝结后,所获血清通过薄膜过滤(罗阿丝虫为8.0μm直径的薄膜,常现丝虫为5.0μm)分离微丝蚴,按改良的Lowrie法冰冻保存。冷冻液为含有50g/L人体白蛋白及10％二甲亚砜的RPMI培养液,该液与0.008M聚 A large number of filarial larvae are the prerequisites for immunological studies on human filariasis. Unfortunately, both Loa and Frequent filariasis have not established a suitable laboratory life history, which greatly hindered the development of both filariasis Progress in immunology. The authors collected microfilariae from the peripheral blood of highly infected patients by a simple, discontinuous, flow-through centrifugation. After coagulation of the specimen, the sera obtained were filtered through a membrane (Lohsiae was 8.0 μm in diameter, 5.0 [mu] m) to separate microfilariae, cryo-preserved according to a modified Lowrie method. The frozen solution was RPMI broth containing 50 g / L human albumin and 10% dimethylsulfoxide, which was mixed with 0.008 M poly