论文部分内容阅读
目的了解肝炎患者中的ＴＴＶ的感染情况并对部分分离株进行基因分型。方法设计通用引物，采用半套式聚合酶链反应检测肝炎患者血清标本中ＴＴＶ ＤＮＡ，并对部分ＰＣＲ产物进行直接测序和序列分析。结果在７２例不同肝炎患者血清中，共检出 ＴＴＶ ＤＮＡ阳性血清 ３９份，总检出率为 ５４． １６％。其中，在非甲～非庚型肝炎患者中，ＴＴＶ ＤＮＡ阳性率为 ８７．５％；在甲～庚型肝炎患者中 ＴＴＶ ＤＮＡ的阳性率（５０．０％）。对４株ＴＴＶ序列分析结果显示，它们与日本分离株ＴＡ２７８、ＮＡ００４、ＴＫＢ５５５、ＰＴ３最高的同源性分别为９７．７％、９９．ｌ％、９６．８％、９１％。经系统发育分析，其中有１株属Ｇ１型，有３株属Ｇ２型。结论本次研究的肝炎人群中，ＴＴＶ感染率较高，感染的 ＴＴＶ分属于 Ｇ１及Ｇ２两个不同的基因型。 ＴＴＶ可能是非甲～非庚肝炎致病因素之一。
Objective To understand the prevalence of TTV infection in hepatitis patients and to genotype some isolates. Methods Universal primers were designed and used to detect TTV DNA in serum from hepatitis patients by semi-nested polymerase chain reaction. Some PCR products were directly sequenced and sequenced. Results Totally 39 TTV DNA positive sera were detected in serum of 72 patients with different hepatitis, with a total detection rate of 54. 16%. Among them, the positive rate of TTV DNA was 87.5% in patients with non-hepatitis A and non-hepatitis G, and the positive rate of TTV DNA in patients with hepatitis A and G (50.0%). The results of the four TTV sequences showed that the highest homologies with the Japanese isolates TA278, NA004, TKB555 and PT3 were 97.7% and 99%, respectively. l%, 96.8%, 91%. According to the phylogenetic analysis, one of them belonged to G1 type and three belonged to G2 type. Conclusions The prevalence of TTV in this study was high, and the infected TTV belonged to two different genotypes, G1 and G2. TTV may be non-A ~ non-G hepatitis one of the risk factors.