论文部分内容阅读
目的:探讨WTX基因对人胃癌SGC-7901细胞生物学行为的影响。方法:将WTX重组质粒或空载体质粒用Attractene法转染SGC-7901细胞,以无处理SGC-7901细胞为空白对照,检测不同时间e GFP标记的转染效率;RT-PCR法检测WTX m RNA水平;CCK-8法测定细胞增殖情况;流式细胞技术检测转染效率、凋亡及细胞周期的变化。结果:转染WTX基因48 h后,e GFP表达最强,转染效率达(33.10±4.16)%;与空白对照组和空载体组比较,WTX转染组WTX m RNA表达明显升高;细胞增殖能力明显降低,S期细胞明显增多,而G1期和G2/M期细胞减少(均P<0.05)。各组细胞均未见明显的细胞凋亡。结论:WTX可通过诱导S期阻滞抑制胃癌细胞SGC-7901生长,但不影响细胞凋亡。
Objective: To investigate the effect of WTX gene on the biological behavior of human gastric cancer cell line SGC-7901. METHODS: SGC-7901 cells were transfected with recombinant plasmids of WTX or empty vector using Attractene method. The transfected SGC-7901 cells were treated with untreated SGC-7901 cells. The transfection efficiency of eGFP was detected by RT-PCR. The cell proliferation was measured by CCK-8 assay. The transfection efficiency, apoptosis and cell cycle were detected by flow cytometry. Results: After transfected WTX for 48 h, the expression of eGFP was strongest and the transfection efficiency was (33.10 ± 4.16)%. Compared with the blank control group and empty vector group, the WTX m RNA expression in WTX transfected group was significantly increased. Proliferative capacity was significantly reduced, S phase cells increased significantly, while the G1 phase and G2 / M phase cells decreased (P <0.05). No obvious cell apoptosis was found in all groups. Conclusion: WTX can inhibit the growth of gastric cancer cell SGC-7901 by inducing S phase arrest, but it does not affect apoptosis.