本文报告了江苏省１９９４～１９９５年急性弛缓性麻痹（ＡＦＰ）病例的病原学监测结果。共检测３５８例粪便标本，分离到病毒５０株，阳性率为１４．０％。其中检出脊髓灰质炎（脊灰）病毒１７株，阳性率为４．７％。对１９９４、１９９５年分离到脊灰病毒的１３例病人的传代标本，用ＰＣＲ法鉴定型内特征均为疫苗株。初步表明，脊灰散在发病，型别已由１９８９、１９９０年流行时期Ⅰ型为主转向Ⅱ、Ⅲ型，且分离的脊灰病毒均为疫苗株。 This article reports the etiological surveillance of acute flaccid paralysis (AFP) cases in Jiangsu Province from 1994 to 1995. A total of 358 stool specimens were detected and 50 strains were isolated, the positive rate was 14.0%. 17 polio (polio) viruses were detected, the positive rate was 4.7%. Passage of 13 cases of patients with poliovirus isolated in 1994 and 1995, all of them were identified as vaccine strains by PCR. Preliminary evidence shows that the incidence of cerebrospinal fluid dispersion, the type from 1989, the prevalence of 1990 Ⅰ type Ⅱ, type Ⅲ, and the separation of poliovirus are vaccine strains.