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[目的]从蜱标本中分离到3株Roseomonas(玫瑰单胞菌),了解其与国内及国外的Roseomonas(玫瑰单胞菌)分离株之间的基因型别关系。[方法]鉴于国内外还没有对Roseomonas(玫瑰单胞菌)基因分型的成型方法可以利用,采用了传统的基于16SrDNA的方法进行不同分离株的比较。[结果]3分离株XTD509、XTD510、XTD622在系统进化树中聚在同一簇内,且与美国分离株R.cervicalisATCC49957聚在一个小的分支内,同源性高达99.1%;与我国另2分离株JS018、TH-G33在系统进化树位于大的不同分支。[结论]该3分离株属于同一基因型,且可能与菌株R.cervicalisATCC49957为同一个种。
[Objective] Three strains of Roseomonas were isolated from tick specimens and their genotypic relationships with domestic and foreign Roseomonas isolates were studied. [Method] In view of the fact that no morphological typing method of Roseomonas was available at home and abroad, 16SrDNA-based methods were used to compare different isolates. [Result] The three isolates XTD509, XTD510 and XTD622 clustered in the same phylogenetic tree and were clustered in a small branch with R.cervicalis ATCC49957 from the United States. The homology was 99.1% Strains JS018, TH-G33 are located in different phylogenetic tree branches. [Conclusion] The three isolates belong to the same genotype, and may be the same strain as R.cervicalis ATCC49957.