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目的:探讨含表皮生长因子和卵泡抑素结构域的跨膜蛋白(TPEF)、信号转导子和转录激活子1(STAT1)在大肠癌中的表达水平及其意义。方法:采用SYBR Green I荧光定量RT-PCR技术对50例大肠癌组织和相应正常大肠黏膜组织中的TPEFmRNA及STAT1 mRNA的表达进行检测,并分析其表达情况与临床病理资料的相关性。结果:癌组织中TPEFmRNA及STAT1 mRNA的相对表达水平明显低于相应正常组织(P<0.05);肿瘤TNM分期中T3/T4癌组织表达水平明显低于T1/T2癌组织(P<0.05);合并淋巴结转移的组织中表达水平亦低于无淋巴结转移者(P<0.05);TPEFmRNA与STAT1mRNA的表达水平呈正相关(r_(癌组织)=0.537,r_(正常组织)=0.851,P<0.001)。结论:TPEF、STAT1基因在大肠癌组织中低表达,与大肠癌的转移相关,可作为大肠癌恶性潜能的检测指标之一。
Objective: To investigate the expression and significance of transmembrane protein (TPEF), signal transducers and activator of transcription 1 (STAT1) in human colorectal cancer including epidermal growth factor and follistatin. Methods: The expressions of TPEF mRNA and STAT1 mRNA in 50 cases of colorectal carcinoma and normal colorectal mucosa were detected by SYBR Green I fluorescent quantitative RT-PCR. The correlation between the expression of TPEF mRNA and STAT1 mRNA was analyzed. Results: The relative expression level of TPEF mRNA and STAT1 mRNA in cancer tissue was significantly lower than that in corresponding normal tissue (P <0.05). The expression level of T3 / T4 in TNM stage was significantly lower than that in T1 / T2 cancerous tissue (P <0.05). The expression level of TPEFmRNA and STAT1mRNA was positively correlated with the expression of STAT1mRNA (r_ (cancer) = 0.537, r_ (normal) = 0.851, P <0.001) . Conclusion: The expressions of TPEF and STAT1 are low correlated with the metastasis of colorectal cancer and may be used as one of the indicators of malignant potential of colorectal cancer.