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目的探讨上海市汉族人群对乙肝疫苗(hepatitis B vaccine,Hep B)免疫应答与人类白细胞抗原(human leukocyte antigen,HLA)-DRB1等位基因的关系。方法 600名上海市汉族人群检测五项HBV血清学标志物,对其中全为阴性的237人以常规程序接种Hep B后,采用聚合酶链反应序列特异性引物(polymerase chain reaction sequence specific primers,PCR-SSP)分型技术,对32例接种Hep B后呈低、无应答者及54例正常应答者的等位基因进行HLA-DRB1检测。结果两组观察对象性别、年龄差异均无统计学意义(均有P>0.05)。低、无应答组HLADRB1*07的频率(16.67%)高于正常组(0.00%)、HLA-DRB1*09的基因频率(37.50%)高于正常应答组(5.56%),差异均有统计学意义(均有P<0.05);HLA-DRB1*04的频率(3.13%)低于正常应答组(20.37%),差异有统计学意义(P=0.022)。而其他HLA-DRB1基因的频率差别均无统计学意义。结论上海汉族人群Hep B低、无应答与HLA-DRB1有关,HLA-DRB1*04、DRB1*07、DRB1*09可能为低、无应答的易感基因。
Objective To investigate the relationship between the immune response of hepatitis B vaccine (Hep B) and the human leukocyte antigen (HLA) -DRB1 allele in Shanghai Han population. Methods A total of 600 HBV serological markers were detected in 600 Han population in Shanghai. Of them, 237 were all negative, Hep B was inoculated by routine procedure, and PCR-specific primers (polymerase chain reaction sequence specific primers) - SSP) typing technique, 32 HLA-DRB1 alleles were detected in 32 low, non-responders and 54 normal responders after Hep B vaccination. Results There were no significant differences in gender and age between the two groups (all P> 0.05). The frequency of HLADRB1 * 07 in low and no response groups was higher than that in normal group (0.006%), while the frequency of HLA-DRB1 * 09 gene was higher in normal subjects (37.50%) than that in normal responders (5.56%) (P <0.05). The frequency of HLA-DRB1 * 04 (3.13%) was lower than that of the normal control group (20.37%), the difference was statistically significant (P = 0.022). The frequencies of other HLA-DRB1 genes were not statistically different. Conclusion Hep B is low and no response is associated with HLA-DRB1 in Shanghai Han population. HLA-DRB1 * 04, DRB1 * 07 and DRB1 * 09 may be low and non-responsive susceptibility genes.