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目前认为治疗细菌性感染,抗生素对吞噬细菌的作用非常重要。抗生素能否渗入细胞内杀伤细菌,不少学者已经用粒细胞作过研究,并证明了青霉素 G 在粒细胞内没有活性。但至今未见青霉素 G 对单核细胞吞噬的金黄色葡萄球菌的作用的报道。为此,作者进行了如下研究。作者从健康自愿者血液中分离单核细胞。用每毫升含0.5单位的肝素洗四次,再用HBSS 把单核细胞稀释成10~7浓度的悬液。金黄色葡萄球菌(42D 型)用含10%的AB 型血清悬液,在37℃孵育30分钟,而后将细菌在1500g 离心10分钟,用冰冷的 HBSS明胶洗两次后,用 HBSS 明胶把金黄色葡萄球菌稀释为每毫升含10~7的菌液。检测青霉素 G 对吞噬细胞吞噬金黄色葡萄球菌的作用。将每毫升含10~7预先处理的金黄色葡萄球菌和每毫升含10~7的单核细胞一起,37℃孵育3分钟后,在冰浴中冷却迅
Now that the treatment of bacterial infections, the role of antibiotics on phagocytic bacteria is very important. Antibiotics can penetrate into the cells to kill bacteria, many scholars have used granulocytes as a study, and proved that penicillin G in the granulocytes no activity. But so far no penicillin G monocytic phagocytosis of the role of the Staphylococcus aureus reported. To this end, the author conducted the following research. The authors isolated monocytes from the blood of healthy volunteers. The cells were washed four times with 0.5 units of heparin per ml, and then monocytes diluted with HBSS to a 10 to 7 concentration suspension. Staphylococcus aureus (type 42D) was incubated with 10% suspension of type AB in serum for 30 minutes at 37 ° C. The bacteria were then centrifuged at 1500g for 10 minutes, washed twice with ice-cold HBSS gelatin and treated with HBSS gelatin Staphylococcus aureus diluted to 10 to 7 per ml of bacteria solution. To detect the role of penicillin G phagocytosis of Staphylococcus aureus. Staphylococcus aureus containing 10 to 7 pre-treatments per ml and monocytes containing 10 to 7 per ml were incubated at 37 ° C for 3 minutes and then cooled in an ice bath