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为探讨激光诱发荧光光谱技术区别正常结肠粘膜与结肠、直肠癌的作用，以氮分子激光（３３７ｎｍ）作激发光源，光学多道分析仪为光谱分析手段，测定３７例外科手术切除的正常结肠和结肠癌的激光诱发荧光光谱。光谱分析发现，正常结肠在４６０ｎｍ处有一主峰，在４００ｎｍ处有一次峰，主峰相对强度较结肠癌强，主峰近红光侧下降较快，Ｆ１（４００ｎｍ处强度／５３０ｎｍ处强度）平均为１．２９，Ｆ２（主峰相对强度）平均为１２０２８，Ｆ３（主峰）在４６０ｎｍ。结肠癌主峰在４７０ｎｍ，次峰在３９０ｎｍ，主峰相对强度较正常结肠弱，主峰近红光侧下降缓慢，主峰强度为正常结肠强度的１／３～１／５，Ｆ１平均为０．４３，Ｆ２平均为３９７０，Ｆ３在４７０ｎｍ。正常结肠粘膜与结肠、直肠癌的激光诱发荧光光谱有明显区别。据此该项技术有可能成为结肠镜下粘膜活检的导向工具。
To explore the role of laser-induced fluorescence spectroscopy in distinguishing normal colon from colon and rectal cancer, nitrogen molecular laser (337 nm) was used as the excitation source. The optical multi-channel analyzer was used as a spectral analysis method to determine 37 cases of normal colon and surgical resection. Laser-induced Fluorescence Spectra of Colon Cancer. Spectral analysis found that the normal colon has a main peak at 460 nm and a peak at 400 nm. The relative intensity of the main peak is stronger than that of colon cancer, and the main peak falls faster near the red side. F1 (intensity at 400 nm/intensity at 530 nm) averages 1. 29, F2 (main peak relative intensity) averages 12028, F3 (main peak) at 460 nm. The main peak of colon cancer is at 470nm, and the secondary peak is at 390nm. The relative intensity of the main peak is weaker than that of the normal colon, and the main peak falls slowly near the red light side. The main peak intensity is 1/3 to 1/5 of the normal colon intensity, and the average F1 is 0.43, F2. The average is 3970, and F3 is at 470 nm. There is a clear difference in the laser-induced fluorescence spectrum between normal colon mucosa and colon and rectal cancer. Based on this technique, this technique may become a guiding tool for mucosal biopsy under colonoscopy.