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本实验取Wistar大鼠雄、雌各60只,用悬浮于2.5％淀粉液内的TNT研碎,以1ml/100g灌胃,一次染毒后观察10天,死亡鼠随即取肝。至第11天中毒未死及对照组鼠全部处理并取肝作光镜和超微结构观察。结果表明TNT对雌鼠LD_(50)为1663.8mg/kg发现,雄鼠为1837.8mg/kg。肝脏除发现变性、坏死及线粒体破坏外,低剂量中毒鼠约50％出现胆小管淤胆和肝细胞核畸变。从而提示TNT可能与变态反应以至致癌作用有关。
In this experiment, 60 male and female Wistar rats were collected and crushed with TNT suspended in 2.5% starch solution. The rats were intragastrically administered at 1 ml/100 g. After 10 days of exposure, the dead rats received liver immediately. To the 11th day of poisoning did not die and control mice were all treated and livers were taken for light microscopy and ultrastructure observation. The results showed that 166.80 mg/kg of LD_(50) was detected by TNT in females, and that of males was 1837.8 mg/kg. In addition to liver degeneration, necrosis, and mitochondrial destruction, approximately 50% of low-dose intoxicated mice developed bile duct cholestasis and hepatocyte nuclear aberrations. This suggests that TNT may be related to allergies and even carcinogenesis.