H-Y抗原是一种次要组织相容性抗原,发现于哺乳类、鸟类、两栖类、硬骨鱼类以及无脊椎动物异配型性别的细胞表商。因其几乎只出现于哺乳动物的雄性,故被认为是哺乳动物的雄性决定物质。为确定分子中决定H-Y血清学活性的因素,先对H-Y阳性细胞用不同方法进行处理以改变分子内的蛋白质或糖结构,然后测试吸收H-Y抗血清的能力。本文旨在证明H-Y抗原的血清学决定因素是糖。为测试H-Y血清学活性对多肽结构的依赖关系,吸收细胞先经胰蛋白酶、a-糜蛋白酶和嗜热菌蛋白酶处理。经酶处理并洗涤后,脾细胞用来吸收H-Y抗血清。该项和继后 H-Y antigen is a secondary histocompatibility antigen found in mammals, birds, amphibians, teleosts, and invertebrate sex-matched cell-surface providers. It is considered to be a mammalian determinant of males because it appears only in males of mammals. To determine the molecular determinants of H-Y seropositivity, H-Y positive cells are first treated differently to alter intramolecular protein or sugar structure and then tested for ability to absorb H-Y antisera. This article aims to demonstrate that the serological determinant of H-Y antigen is sugar. To test the structural dependence of the H-Y serological activity on the polypeptide, the absorbing cells were first treated with trypsin, a-chymotrypsin and thermolysin. After enzyme treatment and washing, spleen cells are used to absorb H-Y antiserum. The item and the following