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本刊1983年第4期发表的刘宗明同志《制剂中硝酸毛果芸香碱的比色测定法》一文中所采用的比色方法,根据我们实践和体会,对该文的比色方法提出如下补充。 1.显色剂溴甲酚绿试液pH值在4.0时,溴甲酚绿与硝酸毛果芸香碱所生成的络合物在氯仿中吸收度虽最大,但吸收度与浓度成正比关系的直线(A-C直线)不能通过原点。而采用pH值为4.4的显色剂时就可克服上述缺点,可绘成吸收度与浓度成正比通过原
The colorimetric method adopted by Liu Zongming in the article No. 4, 1983, “Colorimetric Assay of Pilocarpine Nitrate in Preparations”, based on our practice and experience, supplements the colorimetric method of the article as follows. 1. Bromocresol green reagent color reagent pH value of 4.0, bromocresol green and the complex of nitric acid and pilocarpine generated in chloroform, the absorption of the largest, but the absorption and concentration is proportional to the linear (AC Straight line) can not pass through the origin. The use of pH value of 4.4 when the developer can overcome the above shortcomings can be drawn as the concentration and absorbance is proportional to the original