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目的研究野马追提取物对脂多糖(LPS)诱导的小鼠急性肺损伤(ALI)的保护作用及其机制。方法采用气管滴注LPS法复制小鼠急性肺损伤模型,测定小鼠的肺湿/干重比、血清中补体3(C3)、肺泡灌洗液(BALF)中NO、蛋白质、肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)、白介素-1β(IL-1β)的含量和肺组织中髓过氧化酶(MPO)、超氧化物歧化酶(SOD)的活性,并观察肺部的病理组织学变化。结果野马追提取物可减轻ALI小鼠的肺水肿和肺组织病理改变,降低BALF中NO、蛋白质、TNF-α、IL-6和IL-1β的含量,提高肺组织中SOD的活性,抑制MPO的活性,减少血清中C3的含量并改善肺组织中补体片段C3c的沉积。结论野马追提取物对小鼠急性肺损伤具有保护作用,其作用机制可能与抑制炎症因子、降低C3的水平和抗氧化有关。
Objective To study the protective effect of Mustang Chai extract on lipopolysaccharide (LPS) -induced acute lung injury (ALI) in mice and its mechanism. Methods The model of acute lung injury was induced by tracheal instillation of LPS. The lung wet / dry weight ratio, serum complement 3 (C3), NO, protein, tumor necrosis factor - α, TNF-α, IL-6 and IL-1β, and the activities of myeloperoxidase (MPO) and superoxide dismutase (SOD) Observe the histopathological changes in the lungs. Results Mustang chai extract can reduce pulmonary edema and lung tissue pathological changes in ALI mice, reduce the content of NO, protein, TNF-α, IL-6 and IL-1β in BALF, increase the activity of SOD in lung tissue, Activity, decrease the content of C3 in serum and improve the deposition of complement C3c in lung tissue. Conclusion The extract of Mustang Chai has protective effect on acute lung injury in mice. Its mechanism may be related to the inhibition of inflammatory cytokines, reduction of C3 levels and antioxidation.