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一、引言 1963年3月3日,我场温室内饲养的牛蛙蝌蚪有5千余尾迁移室外水泥池中饲养,不久(3月20日)就发现池中蝌蚪躯干部及尾部出现大量毛状物,尤以鳃孔为多,肉眼看来好象成团的棉花,颜色由白逐渐变成浅黄色,许多蝌蚪开始死亡,经显微镜观查鉴定及死蝌蚪的解剖确定其死亡原因为感染水霉菌造成。为了抢救病蝌蚪,采用高锰酸钾、孔雀石绿(Malaehite-Green)、小苏打和食盐溶液消毒,由于这方面资料的缺乏,在实验中得到一些经验教训,进而寻出不同浓度的高锰酸钾等在不同浸洗时间内感染水霉菌蝌蚪的影响,特作报告,将点滴收获提出,以供有关部门参考。二、材料和方法来源1963年3月从六号水泥池中采取病蝌蚪,一般体长为4—6.5厘米。观察直接取下病蝌蚪体表被病原感染的部分在显微镜下观察,解剖病死的蝌蚪,了解其内腔病变。
I. INTRODUCTION On March 3, 1963, there were more than 5 thousand tadpoles of bullfrogs reared in the greenhouse in our farm. After a short while (March 20), a large amount of hair appeared on the trunk and tail of the tadpoles in the pond Things, especially in the gill hole, seems to the naked eye into a group of cotton, the color from white to pale yellow, many tadpoles began to die, the microscopic examination and dead tadpole anatomy to determine the cause of death as infected water Mold caused. In order to rescue the sick tadpoles, we used potassium permanganate, Malaehite-Green, baking soda and salt solution to disinfect. Due to the lack of information in this field, we got some experiences and lessons in the experiment, and then found different concentrations of high manganese Potassium and so on in different immersion time infected with fungal tadpole impact, special report, the drop will be made for reference for the relevant departments. Second, the source of materials and methods March 1963 taken from the sixth pool of sick tadpoles, the general body length of 4-6.5 cm. Observed directly removed sick tadpole body surface was infected with the part of the observation under a microscope, anatomized dead tadpoles, to understand the lumen lesions.