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目的:以支气管上皮细胞和嗜酸粒细胞共培养为实验模型,探讨不同培养方式对IL-6释放的影响.方法:将支气管上皮细胞和嗜酸粒细胞分别单独培养、接触共同培养、分隔共同培养(用分隔膜将两种细胞分隔在上、下两个池中不接触培养,两池中的培养液可以自由通过分隔膜孔)和在Ma-trigel中共同培养.两种细胞在不同培养条件下释放的IL-6应用酶联免疫吸附试验方法定量.结果:支气管上皮细皮与嗜酸粒细胞在同一培养孔中分隔共同培养时其IL-6释放量远高于两种细胞分别在两个培养孔中独自培养后上清液中IL-6之和[(388±40)ng/Lvs(107±10)ng/L,P<0.001].而两种细胞在同一培养孔中(没有隔膜分隔)接触共同培养,其IL-6释放量又远高于其分隔培养[(1270±131)ng/Lvs(388±40)ng/L,P<0.001];将上述两种细胞在Matrigel中共培养,其IL-6释放又显著高于其在非Matrigel中的培养[(2076±165)ng/Lvs(1270±131)ng/L,P<0.01].结论:支气管上皮细胞与嗜酸粒细胞共同培养过程中可受到相互接触和其在培养过程中所释放的某些物质的活化,从而增加多功能细胞因子IL-6的释放.
OBJECTIVE: To investigate the effects of different culture methods on the release of IL-6 in bronchoalveolar epithelial cells and eosinophils.Methods: Bronchial epithelial cells and eosinophils were cultured separately and contacted with co-culture to separate them Culture (separating the two cells in the upper and lower cells with a separation membrane, without contacting the cells in the two pools, and free passage of the culture liquid in the two cells through the separation membrane) and co-culture in Ma-trigel. The release of IL-6 was quantified by enzyme-linked immunosorbent assay.Results: The release of IL-6 in bronchial epithelium and eosinophil were much higher than those in two kinds of cells The sum of IL-6 in the supernatants after culturing alone in both culture wells [(388 ± 40) ng / L vs (107 ± 10) ng / L, P <0.001] (1270 ± 131) ng / Lvs (388 ± 40) ng / L, P <0.001]. The above two kinds of cells were cultured at Matrigel co-cultured with IL-6 release was significantly higher than its non-Matrigel culture [(2076 ± 165) ng / Lvs (1270 ± 131) ng / L, P <0.01] .Conclusion: Bronchial epithelial cells and eosinophils co-cultured with each other may be exposed to each other and the activation of certain substances released during culture, thereby increasing the expression of multi-functional cytokines IL-6 freed.