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目的：观察严重烫伤大鼠脑、心、肾组织超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH—PX）、乳酸脱氢酶（LDH）、丙二醛（MDA）、ATPase含量的变化及绞股蓝总皂甙（GP）防治作用。方法：选用健康Wistar大鼠85只，随机分成①正常对照组（5只）；②烫伤对照组（40只，10％水合氯醛腹腔麻醉，颈、背部脱毛，浸入100℃沸水中16s造成40％Ⅲ度烫伤）；③烫伤治疗组（40只，烫伤后立即腹腔注射GP（50mg／kg），以后每8小时重复一次）。烫伤后分别于4、8、24、48h几个不同时相点（每个时相点10只）取出脑、心、肾组织分别制成匀浆，测定SOD、GSH—PX、LDH、MDA、ATPase的含量。结果：烫伤后脑、心、肾组织中SOD、GSH—PX、ATPase含量呈进行性下降，LDH、MDA的含量明显上升，各时相点与正常对照组比较有显著性差异（P＜0．01）。采用绞股蓝治疗后，SOD、GSH—PX、ATPase逐渐回升，LDH、MDA的含量明显下降，各时相点与烫伤对照组比较有显著性差异（P＜0.01）。结论：严重烫伤对脑、心、肾组织有损害作用。胶股蓝能够提高机体的抗氧化能力，降低LDH的释放，抑制脂质过氧化产物的形成，对膜结构有明显保护作用。
Objective: To observe superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), lactate dehydrogenase (LDH) and malondialdehyde (MDA) in brain, heart and kidney of rats with severe thermal injury. Changes in ATPase content and gypenosides (GP) control effect. Methods: 85 healthy Wistar rats were randomly divided into 1 normal control group (5 rats); 2 scald control group (40 rats, 10% chloral hydrate, abdominal anesthesia, neck and back hair removal, immersed in boiling water at 100°C for 16s, resulting in 40 % III degree burns); 3 scald treatment groups (40, immediately after scald injection of GP (50 mg/kg), repeated every 8 hours thereafter). After burns, the brain, heart and kidney were removed and homogenized at several different time points (10 at each time point) at 4, 8, 24, and 48 hours to determine SOD, GSH-PX, LDH, and MDA. ATPase content. Results: The contents of SOD, GSH-PX and ATPase in the brain, heart and kidney after scald were progressively decreased, and the contents of LDH and MDA were significantly increased. There was a significant difference between the time points and the normal control group (P<0.01). ). After treatment with Gynostemma pentaphyllum, SOD, GSH-PX, ATPase gradually increased, and the contents of LDH and MDA decreased significantly. There was a significant difference between each time point and the scald control group (P<0.01). Conclusion: Severe scald can damage brain, heart and kidney tissues. The plastic blue can improve the antioxidant capacity of the body, reduce the release of LDH, inhibit the formation of lipid peroxidation products, and have obvious protective effect on the membrane structure.