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本文提供了一个简便、快速测定血清铁和总铁结合力的检测方法。一次分析用样量为5微升,取一滴指血量已经足够。测定血清铁的浓度范围10～300ppb,线性良好。最低测定量为10ppb,相对标准偏差为0.0012。还提出,血清样在肉眼观察无溶血的情况下,不必去除蛋白,样品稀释后直接进行测定,与Curtius.Ch.H在文章中提及的方法一致。样品不必去蛋白的处理,简化了实验步骤,节省时间。
This article provides a simple and rapid method for the determination of serum iron and total iron binding. An analysis of the sample volume of 5 microliters, take a drop of blood is enough. Determination of serum iron concentration range of 10 ~ 300ppb, good linearity. The minimum assay is 10 ppb and the relative standard deviation is 0.0012. It has also been proposed that serum samples should not have to be removed in the absence of hemolysis by naked eyes, and that the samples should be diluted directly for the determination, consistent with the method described by Curtius. Ch. H in the article. The sample does not have to go to the protein processing, simplifying the experimental steps and save time.