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鸡(Gallus gallus)和鸭(Anas platyrhynchos)是新城疫病毒(Newcastle disease virus,NDV)的主要宿主,但对于NDV感染的反应差异显著,先天性免疫应答在其中起重要作用。为了比较鸭源NDV激活鸡和鸭先天性免疫应答的不同,阐明先天性免疫应答在宿主致病性差异产生中的作用,以SD03(鸭源NDV)对鸡和鸭的致病性比较研究为基础,利用荧光定量PCR对不同时间点外周血中模式识别受体(pattern recognition receptors,PRRs)、细胞因子、抗病毒蛋白和主要组织相容性复合体分子(major histocompatibility complex,MHC)m RNA的表达量进行检测和比较。结果表明,SD03在鸡脾脏中的复制效率高于鸭;免疫基因中Toll样受体7(toll-like receptor 7,TLR-7)、白介素1β(interleukin 1β,IL-1β)、IL-2、IL-6、IL-8、干扰素β(interferonβ,IFN-β)、MHCⅠ和MHCⅡ在鸡和鸭外周血中的表达模式不同且表达量变化趋势差异显著;除IL-2外,所有检测基因在鸡外周血中的表达量明显高于鸭。研究结果在机体水平上展示了NDV诱导鸡和鸭先天性免疫反应的不同,并分析了其在致病性差异产生中的作用,为全面理解NDV对不同禽类的致病机制提供了数据支持。
Gallus gallus and Anas platyrhynchos are the main hosts of Newcastle disease virus (NDV), but their response to NDV infection is significantly different. The innate immune response plays an important role. In order to compare the innate immune responses of NDV-activated chickens and ducks, we elucidate the role of innate immune responses in the pathogenesis of host disease. The pathogenicity of SD03 (NDV duck) to chickens and ducks (PRRS), cytokines, antiviral proteins and major histocompatibility complex (MHC) m RNA in peripheral blood at different time points were detected by real-time fluorescence quantitative PCR. The amount of expression was tested and compared. The results showed that the replication efficiency of SD03 in chicken spleen was higher than that in duck. The levels of toll-like receptor 7 (TLR-7), interleukin 1β (IL-1β), IL- The expression patterns of IL-6, IL-8, IFN-β, MHCⅠ and MHCⅡ were different in chicken and duck peripheral blood. The expression of IL-6, IL-8 and IFN- The expression level in chicken peripheral blood was significantly higher than duck. The results showed that the NDV-induced innate immune response of chickens and ducks was different at the body level, and the role of NDV in pathogenicity was analyzed. The results provide data support for understanding the pathogenesis of NDV in different birds.