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从人工培养冬菇菌丝体中用阳离子交换纤维素层析分离,获得一种具有抗血栓形成作用的蛋白质,分子量为1.75×10~4u,等电点为4.3,给大鼠iv10~(-6)mol1/kg,能完全预防体外血栓形成,IC_(50)为4.4×10~(-7)mol/kg。无论是体内或体外,都具有对抗胶原引起的血小板聚集作用,体外全对抗浓度为7.5×10~(-5)mol/L,其IC_(50)为4×10~(-5)1/L,体内剂量1.25×10~(-5)mol/kg(iv)时,血小板聚集抑制率可达60%,抗血凝的最低浓度为3 ×10~(-6)mol/L。
From the cultivated mushroom mycelia, the protein was separated by cation exchange cellulose and an anti-thrombotic protein was obtained. The molecular weight was 1.75×10~4u, the isoelectric point was 4.3, and the rats were iv10~(-6). )mol1/kg can completely prevent thrombosis in vitro, IC_(50) is 4.4×10~(-7)mol/kg. Whether in vivo or in vitro, it has anti-collagen-induced platelet aggregation in vitro with a full antagonistic concentration of 7.5×10 -5 mol/L and an IC 50 of 4×10 -5 /1/L. With an in vivo dose of 1.25×10 -5 mol/kg (iv), the platelet aggregation inhibition rate was 60%, and the lowest concentration of anticoagulation was 3 × 10 -6 mol/L.