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在盆栽模拟复垦的条件下,利用传统平板计数法与BIOLOG方法相结合研究菌剂与缓释肥配施对土壤微生物生态的影响。结果表明,菌剂与缓释肥配施能显著提高土壤细菌、真菌和放线菌的数量。同时对土壤酶活性也具有有明显的影响。就脲酶而言,由于缓释肥与菌剂的配合施用,抑制了其活性,延长了缓释肥的肥效。通过对磷酸酶的研究发现,由于菌剂的加入促进了磷酸酶的活性,菌剂与缓释肥配施处理的土壤磷酸酶活性显著高于纯化肥处理的。通过多样性指数的测定表明菌剂与缓释肥配施处理土壤微生物群落的Shannon多样性指数、Simpson多样性指数和McIntosh均匀度等各项指标均显著高于其他处理的。说明菌剂与缓释肥配施不但可以提高土壤微生物功能多样性,而且还可以提高土壤微生物群落均匀度,从而可促进土壤微生物生态系统的稳定,提高土壤微生物群落的生态功能。
Under the condition of potted reclamation, the combination of traditional plate counting method and BIOLOG method was used to study the influence of the combination of inoculant and slow release fertilizer on the soil microbial ecology. The results showed that the combination of inoculant and slow release fertilizer could significantly increase the amount of soil bacteria, fungi and actinomycetes. Soil enzyme activity also has a significant impact. In the case of urease, due to the combination of slow-release fertilizer and bactericide, its activity is inhibited and the efficiency of slow-release fertilizer is prolonged. Through the study of phosphatase, it was found that the activity of phosphatase was promoted by the addition of fungicide, and the activity of soil phosphatase was significantly higher than that of pure fertilizer. Through the determination of diversity index, Shannon diversity index, Simpson diversity index and McIntosh evenness index of soil microbial community were all significantly higher than those of other treatments. The results showed that the combined application of fungicide and slow-release fertilizer could not only improve the functional diversity of soil microorganism, but also improve the uniformity of soil microbial community, which could promote the stability of soil microbial ecosystem and enhance the ecological function of soil microbial community.