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Eaton(1944年)首先研究了传染性肝炎补体結合反应。繼而国內外学者也进行了广泛的研究,积累了不少資料。但由于传染性肝炎补体結合試驗在实际应用过程中常有相当比数的血清抗补体反应出現,吉林医大研究传染性肝炎病原学过程中,应用鸡胚、豚鼠肝組織抗原分別对236份及25份血清进行补体結合試驗,血清抗补体者分別占18.2％及8％。笔者应用豚鼠肝脾組織抗原进行补体結合試驗,抗补体血清占18.03％。传染性肝炎血清抗补体反应的出現,是限制本試驗研究和实际应用的因素之一。作者在从事黑热病防治研究工作的前几年,曾与同事采用改良Sachs氏法处理了黑热病血清抗补体反应,获得比較滿意的結果。因而作者又用于传染性肝炎补体結合試驗血清抗补体的处理,初步結果简称滿意,介紹如下以供参考。
Eaton (1944) first studied the infectious hepatitis complement-binding reaction. Then domestic and foreign scholars also carried out extensive research and accumulated a lot of information. However, due to the hepatitis C complement fixation test in the actual application of a considerable proportion of serum anti-complement reaction appears, Jilin Medical University in the process of infectious hepatitis etiology, the application of chicken embryos, guinea pig liver tissue antigens were 236 and 25 Serum complement fixation test, serum anti-complement were accounted for 18.2% and 8%. I use guinea pig liver and spleen tissue antigen binding assay, anti-complement serum accounted for 18.03%. The occurrence of infectious hepatitis anti-complement reaction, is to limit the experimental study and practical application of one of the factors. A few years ago, when he was engaged in research on prevention and treatment of kala-azar, he and his colleagues used improved Sachs’ method to deal with the anti-complement reaction of kala-azar serum and obtained satisfactory results. Thus the author is also used for the treatment of infectious hepatitis with complement fixation test serum anti-complement, the initial results referred to as satisfaction, introduced below for reference.