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目的建立田基黄中槲皮素-7-O-α-L-鼠李糖苷的含量测定方法,为田基黄药材的质量控制和综合开发利用提供一定的科学依据。方法采用HPLC法。色谱柱为Hypersil C18柱(250 mm×4.6 mm,5μm),流动相为体积分数0.04%的乙腈-磷酸水(体积比77∶23),流速为1.0 mL.min-1,检测波长为371 nm,柱温为35℃。结果槲皮素-7-O-α-L-鼠李糖苷质量浓度在3.12~99.84 mg.L-1(r=0.9998,n=6)内与峰面积呈良好的线性关系;平均回收率为99.1%(RSD=1.5%,n=9)。结论该方法简便、快速、精密度好,可用于田基黄的质量控制。
Objective To establish a method for the determination of quercetin-7-O-α-L-rhamnoside in Tianji Yellow, which provides a scientific basis for the quality control and comprehensive development and utilization of Tian Jihuang. Method using HPLC method. The column was Hypersil C18 column (250 mm×4.6 mm, 5 μm). The mobile phase was acetonitrile-phosphate water (volume ratio 77:23) with a volume fraction of 0.04%. The flow rate was 1.0 mL.min-1. The detection wavelength was 371 nm. Column temperature is 35°C. Results The mass concentration of quercetin-7-O-α-L-rhamnoside in the range of 3.12 to 99.84 mg.L-1 (r=0.9998, n=6) showed a good linear relationship with the peak area; the average recovery was 99.1% (RSD=1.5%, n=9). Conclusion This method is simple, rapid, and accurate. It can be used for quality control of Tianjihuang.