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目的 对 6株中国人类免疫缺陷病毒 1型 (HIV 1)B/C重组病毒的完整 gag基因和部分pol基因进行序列分析 ,从基因水平上分析是否存在新模式的B/C重组病毒并与其母本毒株进行比较研究 ,尝试对其不同的生物学表型进行解释。方法 从确诊的HIV感染者的全血样本中 ,提取样本基因组DNA ,经套式聚合酶链反应 (PCR)扩增后 ,将扩增产物进行纯化和测序。然后将所得序列进行系统进化树和氨基酸变异分析。用Simplot软件进行序列重组分析并确定重组断点区域 ;用MEGA软件按断点分段做基因进化树分析以验证该断点的正确性 ;用GCG软件包的Distance程序计算基因距离。并分析所研究的HIV 1B/C重组毒株长 2 5 5 0bp基因区段的分区段的基因离散率及基因重组对其功能可能造成的影响。结果 新疆 5份样本均未发现重组断点的变化 ,而重庆 1份样本的逆转录酶区内的B/C断点发生了 16 0个核苷酸的移动。氨基酸序列分析显示 ,我国流行的B/C重组株与其母本B、C毒株之间发生第 2 86位 (R→K/N)和第 799位 (A→T)的变化。结论 我国现在流行的HIV 1B/C重组病毒仍以CRF0 7 BC和CRF0 8 BC两种模式为主 ,在本研究涉及的基因区内尚未发现新模式重组毒株的流行。初步分析表明我国B/C重组毒株 2 86位和 799位氨基酸的变异可能为B/C重组株在我?
Objective To analyze the sequence of the complete gag gene and partial pol gene of 6 strains of human immunodeficiency virus type 1 (HIV 1) B / C virus and analyze whether there is a new pattern of B / C recombinant virus at the gene level and compare it with its parent The strains were compared and tried to explain their different biological phenotypes. Methods Genomic DNA was extracted from whole blood samples of confirmed HIV-infected persons and amplified by PCR. After amplification, the amplified products were purified and sequenced. The resulting sequences were analyzed by phylogenetic tree and amino acid variation. Sequence recombination analysis was performed with Simplot software to determine recombination breakpoint regions; MEGA software was used to segment phylogenetic tree by breakpoint to verify the correctness of the breakpoints; and Distance program was calculated using the Distance program of the GCG software package. The genetic divergence rate of the segmental segments of the 2550 bp gene segment of HIV 1B / C recombinant strains studied and the possible impact of gene recombination on their function were analyzed. Results No changes in recombination breakpoints were found in all five samples in Xinjiang, but a shift of 160 nucleotides in B / C in reverse transcriptase region of Chongqing was detected. Amino acid sequence analysis showed that the 867th (R → K / N) and 799th (A → T) changes occurred between the B / C recombinant strains and their female parent B and C strains. Conclusions The two HIV / AIDS-reovirus strains that are currently circulating in China are still predominantly CRF0 7 BC and CRF0 8 BC. No new pattern of recombinant strains has been found in the gene regions involved in this study. Preliminary analysis showed that the variation of amino acids at positions 866 and 799 of B / C recombinant strains in China may be B / C recombinant strains in me?