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目的采用DNA条形码序列对9种常见的蒿属药用植物进行鉴定,为常见蒿属药用植物的鉴定提供分子依据。方法对9种常见蒿属药用植物的4条候选DNA条形码序列(ITS2、rbcL、matK、psbA-trnH)进行PCR扩增和测序,比较各序列的扩增和测序效率,应用BLAST1、Distance方法来评估各序列的鉴定效率。此外,基于MEGA5分析9种常见蒿属药用植物ITS2序列种间K2P遗传距离并构建NJ树。结果除matK外,其余3条片段的PCR扩增和测序效率均为100%,ITS2序列对9种蒿属药用植物的物种水平鉴定成功率最高,为100%,而psbA-trnH、rbcL、matK、matK+rbcL的鉴定成功率(BLAST1法)分别为83.3%、66.7%、54.5%、75%。通过ITS2序列的种间K2P遗传距离及NJ树均能将不同物种全部区分。结论 ITS2序列可以作为鉴定蒿属药用植物的潜在条形码。
Objective To identify 9 common medicinal plants of Artemisia by DNA barcode sequence and to provide molecular evidences for the identification of common medicinal plants of Artemisia. Methods Four candidate DNA barcode sequences (ITS2, rbcL, matK, psbA-trnH) from nine common medicinal plants of Artemisia were amplified by PCR and sequenced. The amplification and sequencing efficiency of each sequence were compared. The BLAST1 and Distance methods To assess the efficiency of the identification of each sequence. In addition, based on MEGA5 analysis of 9 common artemisia species ITS2 sequences between species genetic distance and NJ tree construction. Results The efficiency of PCR amplification and sequencing of the remaining three fragments except for matK was 100%. ITS2 sequence had the highest success rate of 100% for the identification of nine species of Artemisia medicinal plants, while psbA-trnH, rbcL, The matK, matK + rbcL identification success rate (BLAST1 method) were 83.3%, 66.7%, 54.5%, 75%. The genetic distance between species and the NJ tree through ITS2 sequences all can distinguish different species completely. Conclusion The ITS2 sequence can be used as a potential barcode for the identification of medicinal plants of Artemisia.