自从1989年美国Chiron公司的Kuo等人首先建立了检测抗HCV抗体的第一代ELISA方法(C100-3)以来,第二代ELISA(2nd-ELISA、C200)和确证实验——重组免疫印迹试验(RIBA)又相继问世。还有学者尝试用荧光免疫方法做HCV抗原的检测。上述方法所存在的共同问题是灵敏度与特异性都不十分理想,因此不能作为丙型肝炎早期诊断和最终判断传染性的依据。相比之下,基因诊断却可以解决上述问题。 Since the first generation of ELISA assay (C100-3) to detect anti-HCV antibodies was first established by Kuo et al. Of Chiron Corporation in 1989 in the United States, the second-generation ELISA (2nd-ELISA, C200) and confirmatory test-recombinant immunoblotting (RIBA) have come out. Some scholars try to use fluorescent immunization methods to detect HCV antigens. The common problem existing in the above methods is that the sensitivity and specificity are not very ideal and therefore can not be used as the basis for the early diagnosis and finally the judgment of infectiousness of hepatitis C. In contrast, genetic diagnosis can solve the above problems.