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本文作者用蚀斑形成试验对麻疹疫苗效力进行了研究,发现病毒接种量和不同病毒株对培养温度的敏感程度是影响病毒滴度的关键.作者分别用不同来源的Vero细胞和四种麻疹疫苗(AIK-C、Schwarz FF8、CAM70和TD97)及日本“内部”标准疫苗T-1988.8进行蚀斑试验.此外,分别用不同孔径的培养板进行比较.将疫苗病毒作10倍倍比稀释,室温下吸附60分钟,加含琼脂的培养基覆盖,分别在33℃、35℃和37℃于5%CO_2条件下,倒置培育5～7天后加含中性红的琼脂培养基覆盖并计数,观察到第13天,每天计算蚀斑形成单位(PFU)/ml.
The authors used the plaque formation test to study the efficacy of measles vaccine and found that the virus inoculum size and sensitivity of different virus strains to culture temperature are the key factors affecting virus titer.The authors used different sources of Vero cells and four measles vaccine (AIK-C, Schwarz FF8, CAM70 and TD97) and Japan’s “internal” standard vaccine T-1988.8 were used for plaque testing, respectively, compared with plates of different pore sizes. The vaccine virus was diluted 10 times in multiples and room temperature Under the condition of adsorption for 60 minutes and agar-containing medium, respectively, under the conditions of 33 ° C., 35 ° C. and 37 ° C. under 5% CO 2 for 5 to 7 days, invert agar medium containing neutral red and covered and counted By Day 13, plaque forming units (PFU) / ml were calculated daily.