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建立一种竞争性固相放射免疫分析法（ＳＰ－ＲＩＡ），用于定量测定人体血清或脑脊髓液（ＣＳＦ）中的Ｃ－反应蛋白（Ｃ－ｒｅａｃｔｉｖｅＰｒｏｔｅｉｎ，ＣＲＰ）。最小可测量为５ｎｇ／ｍｌ；批内ＣＶ为４．９％，批间ＣＶ为１２．６％。６８份健康人血清的ＣＲＰ含量为０．０２８～３．４００μｇ／ｍｌ（中位数０．１６６μｇ／ｍｌ）；１６份新生儿脐带血清的ＣＲＰ含量为０．０１３～９．０００μｇ／ｍｌ（中位数０．０９５μｇ／ｍｌ）；６３份“流脑”病人血清ＣＲＰ为０．１５０～５２０．０００μｇ／ｍｌ（中位数７７．５００μｇ／ｍｌ），相当于健康人的４００～５００倍。ＧＳＦ中也可检出低水平的ＣＲＰ（中位数２．０７０μｇ／ｍｌ）。“流脑”急性期血清的ＣＲＰ含量，比恢复期高出３００多倍，而婴幼儿轮状病毒性腹泻病人的急性血ＣＲＰ含量不高（中位数５．５８０μｇ／ｍｌ），且同恢复期血清ＣＲＰ含量之比，仅为２～３∶１，提示此种病毒性感染的急性相应答（Ａｃｕｔｅｐｈａｓｅｒｅｓｐｏｎｓｅ）不如细菌性脑膜炎显著。
A competitive solid-phase radioimmunoassay (SP-RIA) was established for the quantitative determination of C-reactive protein (CRP) in human serum or cerebrospinal fluid (CSF). The smallest measurable was 5 ng / ml; the intra-assay CV was 4.9% and the inter-assay CV was 12.6%. The serum levels of CRP in 68 healthy volunteers ranged from 0.028 to 3.400 μg / ml (median, 0.166 μg / ml). The CRP levels in 16 neonates with umbilical cord serum ranged from 0.013 to 9.000 μg / ml Median 0.095μg / ml); 63 patients with “meningitis” serum CRP was 0.150 ~ 520.000μg / ml (median 77.500μg / ml), equivalent to 400 to 500 times the healthy people. Low levels of CRP were also detectable in the GSF (median 2.070 μg / ml). CRP levels in acute “meningitis” serum were more than 300-fold higher than those in convalescent periods, whereas acute blood levels of acute blood in infants with rotavirus diarrhea were low (median 5.580 μg / ml) and were associated with recovery Serum CRP content ratio, only 2 ~ 3: 1, suggesting that the acute phase of this virus infection response (Acutephaseresponse) is not as significant as bacterial meningitis.