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目的分析河南省甲型H1N1流感病毒神经氨酸酶(NA)基因的变异情况,了解其变异现状及特点。方法对35株甲型H1N1流感病毒毒株提取病毒总RNA,设计引物运用RT-PCR技术扩增编码NA蛋白的基因序列,测序分析核酸序列并用MEGA4.1软件构建进化树,用BLAST进行比对分析。结果分离株NA基因316位G/A和742位A/G(N端106位V/I和248位N/D)发生变异,2个突变位点同时存在;同时,通过BLAST分析,发现我国多个省份和亚洲其它多个地区病毒株NA基因存在945位A/G和1 338位T/C突变,进化树分析发现,这两个位点的突变可能来自于中国猪-人之间相互感染。结论甲型H1N1流感病毒神经氨酸酶(NA)基因在中国内地传播期间个别核酸位点发生了突变,其氨基酸抗原区有一位点发生变异(106 V/I)。
Objective To analyze the variation of neuraminidase (NA) gene of influenza A (H1N1) virus in Henan Province and to understand its variation status and characteristics. Methods The total RNA was extracted from 35 strains of influenza A (H1N1) virus. The primers encoding the NA protein were amplified by RT-PCR. The nucleic acid sequence was sequenced and the phylogenetic tree was constructed by MEGA4.1 software. analysis. Results The G / A at position 316 and A / G at position 742 (V / I at position 106 and N / D at position 248) in the NA gene of the isolate showed two mutations at the same time. By BLAST analysis, There are 945 A / G and 1 338 T / C mutations in the NA gene of virus strains in many provinces and other Asian regions. Phylogenetic tree analysis showed that the mutations in these two loci may come from the Chinese pig-human infection. Conclusion The mutation of the NA site of the influenza A virus (H1N1) gene in mainland China caused a mutation of 106 amino acid antigen (106 V / I).