论文部分内容阅读
目的:探讨喉鳞状细胞癌(LSCC)组织中脆性组氨酸三联体(FHIT)基因启动子甲基化状态及与FHIT蛋白表达的关系。方法:采用PCRbased酶切甲基化及免疫组织化学SP技术,检测41例LSCC及其相对应的喉正常粘膜(NSE)中FHIT基因启动子甲基化状态及其蛋白表达。结果:在NSE组中,无一例出现FHIT基因启动子甲基化,而在LSCC组中,有24.4%(10/41)出现甲基化,且甲基化状态与病理分级及淋巴结转移有关(P<0.01);NSE组及LSCC组FHIT蛋白阳性率分别为100.0%(41/41)和46.3%(19/41),且与肿瘤TNM分期及淋巴结转移有关(P<0.05);FHIT基因启动子甲基化与蛋白阳性表达有明显的相关性(P<0.01)。结论:FHIT基因启动子甲基化是其基因失活的重要机制之一,在LSCC的发生、发展中起重要作用。
Objective: To investigate the promoter methylation status of FHIT gene in laryngeal squamous cell carcinoma (LSCC) and its relationship with FHIT protein expression. Methods: The methylation status and protein expression of FHIT promoter in 41 cases of LSCC and its corresponding laryngeal normal mucosa (NSE) were detected by PCR-based restriction endonuclease methylation and immunohistochemical SP technique. RESULTS: None of the NSE patients had promoter methylation of FHIT gene, whereas in the LSCC group, methylation was found in 24.4% (10/41), and the methylation status was related to pathological grade and lymph node metastasis P <0.01). The positive rates of FHIT protein in NSE group and LSCC group were 100.0% (41/41) and 46.3% (19/41), respectively, which were correlated with TNM stages and lymph node metastasis (P <0.05) There was a significant correlation between methylation and protein expression (P <0.01). Conclusion: Methylation of FHIT gene promoter is one of the important mechanisms of gene inactivation, which plays an important role in the occurrence and development of LSCC.