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1 材料与方法 1.1 临床资料 我院1996年1月～1998年5月收治的726例病毒性肝炎患者中108例(14.8％)抗-HEV阳性者,临床诊断符合1995年在北京修订的《病毒性肝炎防治方案》诊断标准,死亡病例中6例有死后病理诊断证实。所有病例进行了年龄、性别、转归的统计调查。 1.2 检测方法 所有病人入院后检测肝炎病原学指标,包括抗HAV-IgM、乙型肝炎病毒(HBV)标志、抗-HCV、抗-HEV,均用ELISA法检测。HBV DNA用聚合酶链反应(PCR)及寡核苷酸探针杂交,HCV RNA用PCR法。
1 Materials and Methods 1.1 Clinical data Our hospital from January 1996 to May 1998 726 cases of viral hepatitis in 108 patients (14.8%) were anti-HEV positive clinical diagnosis consistent with the 1995 revision of the Beijing virus Sexual Hepatitis Control Program "diagnostic criteria, 6 cases of death confirmed by pathological diagnosis after death. All cases were carried out by age, gender, outcome of the survey. 1.2 Detection Methods All patients admitted to the hospital after the detection of hepatitis etiological indicators, including anti-HAV-IgM, hepatitis B virus (HBV) markers, anti-HCV, anti-HEV were detected by ELISA. HBV DNA is hybridized by polymerase chain reaction (PCR) and oligonucleotide probes, and HCV RNA is detected by PCR.