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将脂肪酶固定在ｐＨ玻璃电极上，制成甘油三酯酶传感器．对传感器的制作方法和响应性能进行了研究．在３７℃，选择ＴｒｉｓＨＣｌ缓冲溶液（ｐＨ８５）为测量介质，所制传感器对甘油三酯的响应线性范围为３０９×１０－６～１９１×１０－３ｍｏｌ／Ｌ，响应斜率为３２７ｍＶ／ｐＣ，响应时间为５～１０ｍｉｎ，使用寿命可达２５ｄ．用研制的传感器测定了人和兔血清中的甘油三酯含量，结果满意．
The lipase was immobilized on a pH glass electrode to make a triglyceride enzyme sensor. The fabrication method and response performance of the sensor are studied. The linear response range of the sensor to triglyceride was 37.09 × 10-6 ~ 1.9 × 10-3mol / L at 37 ℃ with Tris-HCl buffer (pH 8.5) as the measuring medium. The response Slope of 32 7mV / pC, response time of 5 ~ 10min, the service life of up to 25d. The developed sensor was used to determine the triglyceride content in human and rabbit serum with satisfactory results.