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DNA sequencing analysis in 38 kd phosphorylated protein (pp38) ORF of Marek’s disease viruses (MDV) indicated that all tested 10 virulent strains with different pathotypes had ’A’ at base #320 and glutamine at aa#107 while reacted with monoclonal antibody (Mab) H19 in indirect fluorescence antibody test (IFA). However, vaccine strain CVI988 had ’G’ at base#320 and arginine at aa#107 instead, when it was negative in IFA with Mab H19. Some strains were also reactive with Mab T65 in IFA while there was ’G’ at base #326 and glycine at aa#109, but the other strains, which had ’A’ at base #326 and glutamic acid at aa#109, did not react with Mab T65. By comparison of CVI988 to its point mutants CVI/rpp38(AG) and CVI/rpp38(AA) with 1 or 2 base(s) changes at bases #320 and /or #326 of pp38 gene for their reactivity with Mab H19 and T65, it was confirmed that the glutamine at aa#107 and glycine at aa#109 were critical to epi-topes H19 and T65 respectively. Immuno-reactions to MDV were compared in SPF chickens ino
DNA sequencing analysis in 38 kd phosphorylated protein (pp38) ORF of Marek’s disease viruses (MDV) indicated that all tested 10 virulent strains with different pathotypes had ’A’ at base # 320 and glutamine at aa # 107 while reacted with monoclonal antibody ) H19 in indirect fluorescence antibody test (IFA). However, vaccine strain CVI988 had ’G’ at base # 320 and arginine at aa # 107 instead, when it was negative in IFA with Mab H19. in IFA while there was ’G’ at base # 326 and glycine at aa # 109, but the other, which had ’A’ at base # 326 and glutamic acid at aa # 109, did not react with Mab T65. of CVI988 to its point mutants CVI / rpp38 (AG) and CVI / rpp38 (AA) with 1 or 2 base (s) changes at bases # 320 and / or # 326 of pp38 gene for their reactivity with Mab H19 and T65, it was confirmed that the glutamine at aa # 107 and glycine at aa # 109 were critical to epi-topes H19 and T65 respectively. Immuno-reactions to MDV were compared i n SPF chickens ino