观察免疫荧光时,常在真皮乳头部位出现较强的非特异荧光,这可能是标记FITC的抗体与真皮结构及细胞膜上存在的FC受体结合之故。本文介绍了一种改良的免疫荧光技术可减少直接和间接免疫荧光(IF)技术时非特异的真皮乳头荧光,从而增加了本试验的准确性。具体方法按照标准技术进行。所不同的是在皮肤标本上一组分别加FITC标记的山羊抗人IgG-Fab或FITC标记的山羊抗人IgG(γ链特异的),另一组分别加与等量用PBS1/40稀 Immunofluorescence observation, often in dermal papilla strong non-specific fluorescence, which may be labeled FITC antibodies and dermal structure and cell membrane present on the FC receptor binding it. This article describes a modified immunofluorescence technique that reduces non-specific dermal papillary fluorescence when direct and indirect immunofluorescence (IF) techniques are added, thereby increasing the accuracy of this assay. Specific methods in accordance with the standard technology. Except that a group of goat anti-human IgG-Fab or FITC-labeled goat anti-human IgG (γ chain-specific) respectively labeled with FITC was added to the skin specimens, and the other group was given an equal amount of PBS1 / 40